Cell Lines Service：Personalized overexpression cell lines

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Sanyou constructed stable cell lines expressing target proteins on the cell surface by lentivirus infection, and so far more than 100 stable cell lines are successfully generated. There are immune checkpoint expressing cell lines such as PD-1, PD-L1, CTLA4, Lag3 and Siglec15 and a variety of GPCR expressing cell lines. Overexpression cell lines can be used in FACS experiments during antibody drug development, helping the validations of the candidate antibody drugs. All overexpression cell lines are verified for high protein expression and free of mycoplasma contamination.
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Take the cell line verification of the Human CLDN18.2-HEK293T overexpression cell line as an example.

Example3: Binding activity test
IMAB362 has a high binding activity to Human CLDN18.2-HEK293T overexpression cell line, EC50= 8.359 nM.

Overexpression cell lines has the following advantages:
(1) Standard documents: good traceability and can be used for IND filing;
(2) Stable expression: the target protein can be expressed stably for more than 15 generations;
(3) Safe and reliable: no mycoplasma contamination;

List of example overexpressed cell lines
Promotion code: SY2A-3 (Available from Mar 14^st - May 31^st)

Target Name	Species	Cell Type	Target Name	Species	Cell Type
4-1BB	Human	CHO-s	CX3CR1	Human	CHO-s
B7-H3	Human	CHO-s	CX3CR1	Human	HEK293
B7H3-2Ig	Human	CHO-s	CXCR2	Human	CHO-s
B7-H4	Human	CHO-s	CXCR2	Human	BaF3
BAFFR	Human	CHO-s	CXCR2	Human	HEK293
BCMA	Human	CHO-s	CXCR4	Human	CHO-s
BCMA	Human	HEK293	CXCR4	Human	BaF3
BCMA	Cynomolgus	CHO	CXCR4	Human	HEK293
CCR2	Human	CHO-s	IL-4R	Human	CHO-s
CCR4	Human	CHO-s	LAG-3	Human	CHO-s
CCR4	Human	HEK293	LAG-3	Human	Jurkat
CCR7	Human	HEK293	OX40	Human	CHO-s
CD38	Human	CHO-s	PD-1	Human	CHO-s
CD40	Human	CHO-s	PD-1	Human	Jurkat
CD40	Cynomolgus	CHO-s	PD-L1	Human	CHO-s
CD40	Human	Jurkat	PD-L1	Mouse	CHO-s
CD40	Human	HEK293	PD-L1	Cynomolgus	CHO-s
CD69	Human	Jurkat	PSMA	Human	CHO-s
CD69	Human	Jurkat	Siglec-15	Human	HEK293
CD69	Mouse	CHO-s	Siglec-15	Mouse	HEK293
CD70	Human	CHO-s	Siglec-15	Cynomolgus	HEK293
CD73	Human	CHO-s	Siglec-15	Mouse	HEK293
CD74	Human	CHO-s	Siglec-15	Human	K562
CLDN18.1	Human	HEK293	SIRP-α	Human	CHO-s
CLDN18.2	Human	HEK293	TACI	Human	CHO-s
CLDN18.2	Human	KATOIII	TIGIT	Human	CHO-s
CLDN18.2	Human	NUGC-4	TIGIT	Cynomolgus	CHO-s
CLDN18.2	Mouse	HEK293	TIGIT	Human	Jurkat
CLDN-6	Human	CHO-s	TIM-3	Human	CHO-s
CSF1-R	Human	CHO-s	TIM-3	Cynomolgus	CHO-s
CTLA-4	Human	CHO-s	TIM-3	Human	Jurkat
CTLA-4	Cynomolgus	CHO-s	TIM-3	Mouse	CHO-s
CTLA-4	Human	Jurkat	TLR4	Human	HEK293

If you don’t find what you need in our list

Please send an email to service@sanyoubio.com or contact your customer specialist to request a quote or technical support.

Sincerely.

Qingyang

Customer Specialist
Sanyou Biopharmaceuticals Co., Ltd.
Address: Building 6, No.188 Xinjunhuan Road,
Minhang District, Shanghai, China 201114
Email: service@sanyoubio.com
Tel: +86-21-3368 1627
Website: www.sanyoubio.com