Greetings!
Sanyou Biopharmaceuticals Co., Ltd. is a leading international CRO (contract research organization) company focused on R&D and production of innovative antibody drugs. Since 2018, Sanyou has established an advanced nanobody library platform and launched nanobody library construction and screening services. The purpose is to provide high-affinity and high-specificity nanobodies for the development of antibody drugs.
With this service, customers only need to provide antigen or antigen sequence information and we will conduct alpaca immunization and construction of high-quality alpaca-derived nanobody phage display library. Then through high-throughput screening of the nanobody library, dozens to hundreds of high-affinity nanobodies can be obtained. Sanyou has six quality parameters for the construction of antibody libraries to ensure that the correct expression rate of antibodies and the unique clone rate are close to 100%. The high-efficiency screening of nanobody Library relies on Sanyou's high-throughput automatic screening workstation, which can obtain nanobodies with affinity of up to pM and high specificity.
High-standard antibody library construction
✔High-quality library capacity;
✔The proportion of stop codon <10%;
✔Empty vector ratio < 10%;
✔Unique sequence ratio>90%;
✔The correct antibody display rate>80%;
✔No contamination.
High-quality antibody screening
✔High affinity: antibody affinity as high as pM;
✔High efficiency: high-throughput automatic screening;
✔A variety of screening strategies: flexible screening strategies with protein antigens, peptides and over-expressing cells.
Example: Affinity ranking assay of nanobodies
The figure shows that a number of lead nanobody which are obtained from the nanobody library, with similar affinity or significantly superior affinity as to the benchmark.
List of nanobody library construction and screening targets that have been delivered
| Targets |
| ADAMTS5 |
Amyloid beta |
CD47 |
| CD7 |
CEA |
cMet |
| CX3CR1 |
CXCR2 |
CXCR4 |
| CXCR7 |
DLL4 |
DR5 |
| EGFR |
HER3 |
IgE |
| IL-23 |
Kv1.3 |
PD-1 |
| PD-L1 |
RANKL |
RSV |
| TIM-3 |
TNF-alpha |
Undisclosed |
| VEGF |
Vwf |
| Name |
Content |
Input |
Output |
Period |
| Alpaca immunization and Nanobody library construction service |
Phage library construction
|
Antigen or antigen information |
Immunization library that meets delivery standards |
10-12 weeks
|
| Nanobody library screening service |
Screening for nanobodies
|
Antigen or cell lines |
＞30 lead nanobody |
3-5 weeks
|
Please send an email to service@sanyoubio.com or contact your customer specialist to request a quote or technical support.
Sincerely.
Qingyang
Customer Specialist
Sanyou Biopharmaceuticals Co., Ltd.
Address: Building 6, No.188 Xinjunhuan Road,
Minhang District, Shanghai, China 201114
Email: service@sanyoubio.com
Tel: +86-21-3368 1627
Website: www.sanyoubio.com
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